3/18/2023 0 Comments Savage scaffold![]() ![]() Second, work from our group has demonstrated the inherent genetic modularity of the CCM may all for it to be transferred to other organisms in order to improve their growth (Bonacci et al. 2016) suggests that pH, carbon transport, and carboxysome permeability are highly intertwined and we are interested in mechanisms that can control the permeability of the carboxysome shell. For example, our systems model of the CCM (Mangan et al. Despite numerous structural studies of carboxysome components, there are many open questions we are currently investigating related to how this massive complex self-assembles and functions in the context of the cellular milieu. ![]() Rubisco is a notoriously bad enzyme, and it is thought the inside of the carboxysome provides a microenvironment within the cell of high carbon dioxide (and possibly low oxygen) as a means of improving rate and specificity. Perhaps most amazing is the use of a protein organelle, the carboxysome, to achieve improved activity of Rubisco. Towards a Total Cell Biological Understanding of CO2 Concentrating mechanismsĬyanobacteria carry out photosynthetic carbon dioxide assimilation by controlling when and where these biochemical reactions occur. Our specific areas of research are as follows. Given the generality of these tools, we also explore how engineered genome editors can be used to improve editing in human cells. We are therefore also interested in the development of novel genome editing technologies for accelerating genetic experiments in plants. ![]() Ultimately, we hope to develop a total cell biological understanding of how CCM function emerges from individual components and to use this understanding for the improvement of photosynthetic CO2 fixation in plants. We use the tools of biochemistry, molecular biology, and synthetic biology to identify and characterize the key players and mechanistic principles underlying CCM function. For the past several years, we have focused much of our effort on the bacterial Carbon dioxide Concentrating Mechanism (CCM), a multi-scale, multi-component system that functions to increase the rate and specificity of the critical enzyme ribulose-1,5-bisphosphate carboxylase / oxygenase (Rubisco). We employ several model organisms including various bacterial species, mammalian cells and plant cells. It catalyzes the assimilation of inorganic carbon into the organic world and is the ultimate source of nearly all nutritional calories on Earth. Photosynthesis is uniquely important for human society. We are particularly interested in photosynthetic CO2 fixation, a complex set of reactions that must occur in the right place and time to capture and convert light into stored chemical energy. The goal of the Savage Lab is to understand how protein machinery facilitates the biochemistry of the cell. ![]()
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